Fig. 6

Real-time qPCR. Validation of the 2nd set of microarrays to verify the pooling approach using quantitative real time PCR as independent method. RNA of individual FL2 and Ctrl mice was used prior of pooling for the microarray. Samples were normalized to a combination of reference genes (36B4, GAPDH, HPRT, B2m) and statistically evaluated employing the Relative Expression Software Tool (REST 2009) [12]. Relative expression ratio are presented as log2 bars (a) (* p < 0.05; *** p < 0.001). Numbers below bars indicate fold change (FC) values calculated for the 2nd set of microarrays comprising the same animals as pooled samples. Numbers in brackets are FCs of the 1st set of microarrays as biologically independent replicates. Scatter blot illustrates the FCs acquired by both microarray (abscissae) and qPCR (ordinates), based on the same set of animals (b)