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Fig. 1 | BMC Genomics

Fig. 1

From: Discovering the potential of S. clavuligerus for bioactive compound production: cross-talk between the chromosome and the pSCL4 megaplasmid

Fig. 1

Change of gene expression level and DNA quantification of genes in S. clavuligerus pSCL4- in relation to wild type strain. a Change of expression level of genes in S. clavuligerus pSCL4− chromosome at 46.5 h in relation to the wild type strain. The pattern of the change of gene expression level was the same at 22.5 h and 60 h (not shown). b qPCR of: i) pSCL4 located genes (parB pSCL4) deleted in S. clavuligerus pSCL4−, ii) genes located in plasmids different from pSCL4 (parA pSCL2), iii) genes located in the central part of the chromosome (SCLAV_5146, SCLAV_5308), and iv) genes located in the 303 kb stretch of the right arm of the chromosome (SCLAV_5482, 5485, 5487, 5491, 5521, 5580, 5585, 5692, 5719 and the right telomere (nt 6,760,214 to 6,760,380)

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