Fig. 5

Effects of the lack of pSCL4 on genes with different functions. a The pie chart shows miscellaneous genes affected in S. clavuligerus pSCL4⁻ classified by their role in the cell according with the COG database. The number of genes affected is indicated in parenthesis. b Effect of the lack of pSCL4⁻ and claR in the formation of aerial mycelium and spores. S. clavuligerus ATCC 27064, S. clavuligerus pSCL4⁻ and S. clavuligerus ΔclaR::aac were grown in plates of ME medium during 9 days. Note the extracellular complementation on the formation of aerial mycelium between the wild type strain, or S. clavuligerus pSCL4⁻, and S. clavuligerus ΔclaR::aac, and the lack of effect of S. clavuligerus ATCC 27064 on S. clavuligerus pSCL4⁻