Fig. 6
Western blot analysis of the parthenocarpy-specialized proteins that were actively expressed during NP and CP fruit set. iTRAQ result showed that Csa1M025890.1, Csa4M036590.1, Csa7M073540.1, and Csa7M450640.1 were dramatically increased in natural parthenocarpic fruits, while Csa2M139820.1 was highly increased in cytokinin-induced parthenocarpic fruits (Table 2; marked by solid stars, >5-fold). The expression patterns of these proteins were further analyzed by western blotting. a Expression analysis of the parthenocarpy-specialized proteins during NP and CP fruit set individually. b Expression analysis of the parthenocarpy-specialized proteins in response to hormone treatments in seedlings. The cucumber beta-actin (Csa5M182010.1) was used as reference protein for Western blotting. The experiment was repeated three times. The band intensity analysis of western blots was conducted using ImageJ (Version 1.4), and the data are presented in Additional file 6: Figure S5A. CK: Seedlings without phytohormone treatment; NAA: treated with 50 μM NAA; CPPU: treated with 10 μM CPPU; GA: treated with 10 μM GA3