Fig. 7
Expression analysis of Csa2M059750.1 during different fruit developmental processes and the response to phytohormone treatments. Csa2M059750.1 was considered a candidate parthenocarpy regulatory protein by combined analysis of iTRAQ and genetic mapping results (Wu et al. [25]). The protein expression of Csa2M059750.1 was analyzed by western blotting. a The expression of Csa2M059750.1 during fruit development; b The expression of Csa2M059750.1 after phytohormone treatment in cucumber seedlings. The cucumber beta-actin (Csa5M182010.1) was used as a reference protein for western blotting. The experiment was repeated three times. The band intensity analysis of western blots was recorded using ImageJ (Version 1.4), for which the data are presented in Additional file 6: Figure S5B. CK: Seedlings without phytohormone treatment; NAA1: treated with 5 μM NAA; NAA2: treated with 10 μM NAA; NAA3: treated with 50 μM NAA; CPPU: treated with 10 μM CPPU; GA: treated with 10 μM GA3