Skip to main content
Fig. 3 | BMC Genomics

Fig. 3

From: RNA-seq and Tn-seq reveal fitness determinants of vancomycin-resistant Enterococcus faecium during growth in human serum

Fig. 3

Tn-seq analysis to identify E. faecium genes required for growth in human serum. Bubbles represent genes, and bubble size corresponds to the fold-changes (for visual reasons, a 100-fold change in transposon mutant abundance is set as a maximum) derived from the read-count ratio of libraries grown in BHI to libraries grown in human serum. On the x-axis genes are shown in order of their genomic location and the chromosome and plasmids are indicated. The outcome of statistical analysis of the Tn-seq data is indicated on the y-axis. Genes with a significant change (q < 0.05) in fitness in serum versus BHI are grouped by function and are labelled with different colors, and the name or locus tag and the change in abundance between the control condition and growth in serum is indicated next to the bubbles in parentheses. Negative values indicate that mutants in these genes outgrow other mutants in serum, suggesting that these mutants, compared to the wild-type strain E. faecium E745, have a higher fitness in serum

Back to article page