Fig. 5

Comparison of the strain 34mel genome, plasmid pFBAOT6 from Aeromonas caviae, and transposon Tn1721. The three “panels” represent a transposon Tn1721, b a segment of plasmid pFBAOT6 (pos 83,802–41,003) and c a segment of the strain 34mel genome (pos 4,961,312–4,993,921). The shared segments are connected by gray boxes. In all panels, filled thick arrows indicate regular protein-coding genes, “striped” arrows indicate pseudogenes. Coloring: yellow colors: transposase; brown: resolvase; green colors: tetracycline resistance genes; light-green with dark-green border: methyl-accepting chemotactic protein; red: TOL plasmid related genes. For reasons of clarity, many protein-coding genes are not shown (see Additional file 1: Fig. S5 for more detailed images). a The top panel shows extended transposon Tn1721 and the basic transposon Tn1722 (yellow lines). Inverted terminal repeats are indicated by yellow triangles (not drawn to scale). b The middle panel shows the region from plasmid pFBAOT6 where Tn1721 is interrupted by two insertions. The part of Tn1721, which is also found in the 34mel genome is indicated by the thin blue line right above the yellow line of Tn1721/1722. A 3 kb insertion, which represents a transposon cassette, is drawn as an inserted line: thin orange arrows indicate ISApu1 and ISApu2 positioned in opposite orientation. The target duplication, which encloses the complete cassette, is indicated by orange triangles (not drawn to scale). The central thin line represents a 642 bp segment, which is carried along on the transposon cassette. A 28 kb insertion is drawn “in-line”. This region terminates with a copy of the Tn3-related transposon ISPa38 (drawn in yellow-green with terminal inverted repeats indicated by yellow-green triangles). The region, which is also found in the 34mel genome is indicated by the blue line drawn right above ISPa38 and its transfer is also indicated. A sequence, which is unique to pFBAOT6 is indicated by a thin green line. Three segments, which are shared between pFBAOT6 and the 34mel genome are indicated by two thin blue lines and an interrupted box (not drawn to scale, total length 14855 bp). The 206 bp MITE MITEAeca1, which is found in pFBAOT6 but not in the 34mel genome, is placed above the main sequence line and highlighted in bright green. An 1865 bp sequence, which is unique to pFBAOT6 and is replaced by an iso-positioned resolvase gene in the 34mel genome is drawn in green above the main sequence line. c Transposon TnAs1 is drawn as a dark-red thick line. The element AsIRS12, which terminates with an inverted terminal repeat (yellow triangle) is indicated by a thick grey line. For all other markup see panel (b)