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Table 9 Primers used in this study

From: The insect pathogenic bacterium Xenorhabdus innexi has attenuated virulence in multiple insect model hosts yet encodes a potent mosquitocidal toxin

Primers

5′ to 3′ sequencea

Use

XIS1_460109ApaUpF

NNNNNNGGGCCCCAGGATATGCCATTCAGC

Mutant construction

XIS1_460109BamUpR

NNNNNNGGATCCCAATGACATCAGGCACAC

Mutant construction

XIS1_460109BamDnF

NNNNNNGGATCCGAACCATCGCAGATTGAG

Mutant construction

XIS1_460109XbaDnR

NNNNNNTCTAGAGCCCAATCGCTTCATATC

Mutant construction

XIS1_460115ApaUpF

NNNNNNGGGCCCGAATCGCCCTGGATTATG

Mutant construction

XIS1_460115BamUpR

NNNNNNGGATCCCCCTCTGGCTGATAATAG

Mutant construction

XIS1_460115BamDnF

NNNNNNGGATCCCTCAGGCTCGATTATTGG

Mutant construction

XIS1_460115XbaDnR

NNNNNNTCTAGACTGAATGTACTCCTGCTG

Mutant construction

NilBF

NNNCATATGAGGAAAACGCCACATTCCGG

Confirmation PCR

NilBR

NNNGGGCCCTTGCATGGTTTGGTTG

Confirmation PCR

M13F (−20)

GTAAAACGACGGCCAG

Sequencing PCR

M13R

CAGGAAACAGCTATGAC

Sequencing PCR

  1. aN represents A, T, G or C. Engineered restriction enzyme sites are underlined