Primers | 5′ to 3′ sequencea | Use |
---|---|---|
XIS1_460109ApaUpF | NNNNNNGGGCCCCAGGATATGCCATTCAGC | Mutant construction |
XIS1_460109BamUpR | NNNNNNGGATCCCAATGACATCAGGCACAC | Mutant construction |
XIS1_460109BamDnF | NNNNNNGGATCCGAACCATCGCAGATTGAG | Mutant construction |
XIS1_460109XbaDnR | NNNNNNTCTAGAGCCCAATCGCTTCATATC | Mutant construction |
XIS1_460115ApaUpF | NNNNNNGGGCCCGAATCGCCCTGGATTATG | Mutant construction |
XIS1_460115BamUpR | NNNNNNGGATCCCCCTCTGGCTGATAATAG | Mutant construction |
XIS1_460115BamDnF | NNNNNNGGATCCCTCAGGCTCGATTATTGG | Mutant construction |
XIS1_460115XbaDnR | NNNNNNTCTAGACTGAATGTACTCCTGCTG | Mutant construction |
NilBF | NNNCATATGAGGAAAACGCCACATTCCGG | Confirmation PCR |
NilBR | NNNGGGCCCTTGCATGGTTTGGTTG | Confirmation PCR |
M13F (−20) | GTAAAACGACGGCCAG | Sequencing PCR |
M13R | CAGGAAACAGCTATGAC | Sequencing PCR |