Skip to main content

Table 9 Primers used in this study

From: The insect pathogenic bacterium Xenorhabdus innexi has attenuated virulence in multiple insect model hosts yet encodes a potent mosquitocidal toxin

Primers 5′ to 3′ sequencea Use
XIS1_460109ApaUpF NNNNNNGGGCCCCAGGATATGCCATTCAGC Mutant construction
XIS1_460109BamUpR NNNNNNGGATCCCAATGACATCAGGCACAC Mutant construction
XIS1_460109BamDnF NNNNNNGGATCCGAACCATCGCAGATTGAG Mutant construction
XIS1_460109XbaDnR NNNNNNTCTAGAGCCCAATCGCTTCATATC Mutant construction
XIS1_460115ApaUpF NNNNNNGGGCCCGAATCGCCCTGGATTATG Mutant construction
XIS1_460115BamUpR NNNNNNGGATCCCCCTCTGGCTGATAATAG Mutant construction
XIS1_460115BamDnF NNNNNNGGATCCCTCAGGCTCGATTATTGG Mutant construction
XIS1_460115XbaDnR NNNNNNTCTAGACTGAATGTACTCCTGCTG Mutant construction
NilBF NNNCATATGAGGAAAACGCCACATTCCGG Confirmation PCR
NilBR NNNGGGCCCTTGCATGGTTTGGTTG Confirmation PCR
M13F (−20) GTAAAACGACGGCCAG Sequencing PCR
M13R CAGGAAACAGCTATGAC Sequencing PCR
  1. aN represents A, T, G or C. Engineered restriction enzyme sites are underlined