Fig. 3
Putative lanthipeptide gene clusters in Brevibacterium and Corynebacterium casei LMG S-19264, and structure of the BreLI Island. a Organization of class III lanthipeptide gene clusters. (a) B. antiquum P10, B. antiquum CNRZ 918, B. linens ATCC 9172T and Corynebacterium casei LMG S-19264; (b) B. aurantiacum ATCC 9174; (c) B. linens Mu101; (d) B. mcbrellneri ATCC 49030T; (e) B. aurantiacum CNRZ 920; (f) Streptomyces avermitilis MA-4680 (Avermipeptin, Ave); (g) Catenulispora acidiphila DSM 44928 (Catenulipeptin, Cat); (h) Thermomonospora curvata DSM 43183 (Curvopeptin, Cur); (i) Saccharopolyspora erythraea NRRL 2338 (Erythreapeptin, Ery); (j) Kribbella flavida DSM 17836 (Flavipeptin, Fla); (k) Streptomyces griseus NBRC 13350 (Griseopeptin, Gri); (l) Streptomyces coelicolor A3(2) (SapB); (m) Actinomadura namibiensis DSM 6313 (Labyrinthopeptin, Lab); (n) Actinoplanes sp. NAI112 (NAI-112). b Sequences of class III lanthipeptide precursors. (Me)Lan/(Me)Lab rings are indicated by a blue line, recognition motifs in the leader peptides are indicated by a green line, Pro residues in the leader are indicated by a red box. BreA1 corresponds to the protein product of Ga0063701_00554 (strain P10), Ga0063689_03239 (CNRZ 918), Ga0063694_02885 (ATCC 9172T), ORF1 (ATCC 9174, see Additional file 11 for more details), ORF25 (Corynebacterium casei LMG S-19264); BreA2 corresponds to the protein product of Ga0063701_00562 (P10), Ga0063689_03231 (CNRZ 918), ORF2 (ATCC 9172T) and ORF26 (Corynebacterium casei LMG S-19264); BreB, BreC, BreD1 and BreD2 correspond to the protein products of Ga0063699_00711 (Mu101), HMPREF0183_0898 (ATCC 49030T), Ga0063691_02687 and Ga0063691_02686 (CNRZ 920), respectively. c Schematic map of the BreLI Island in B. antiquum P10. Nucleotide position values refer to contig Ga0063701_102. The lanthipeptide gene cluster region (corresponding to BreA1 and BreA2 for strains P10, CNRZ 918, ATCC 9172T and Corynebacterium casei LMG S-19264, and BreA1 for ATCC 9174) is underlined. The 12-bp sequences are the perfect repeat delimiting the BreLI Island