Table 1 Details of the differentially expressed protein spots in skin mucus of S. aurata after chronic wounds
From: Chronic wounds alter the proteome profile in skin mucus of farmed gilthead seabream
SNa | Protein name | Organism ANb | pI/MWc | S/Cd | Mp/Up e | Peptide sequence and e-valuef |
|---|---|---|---|---|---|---|
H1 | Histone H4 | Oncorhynchus mykiss P62797 | 11.4/11.4 | 76/19 | 2/2 | VFLENVIR (2.9*10−5) TVTAMDVVYALK (0.002) |
H2 | Apolipoprotein A1 | Sparus aurata AAT45246 | 5.3/15.9 | 151/39 | 3/3 | LLNLLSQAQTASGPMVEQASQDGR (0.0068) EYAETLQAKPEFQAFVK (0.025) VATALGEEASPLVDK (0.016) |
H3 | Histone H2B | Danio rerio Q5BJA5 | 10.4/13.6 | 28/7 | 1/1 | LLLPGELAK (0.0016) |
H4 | Cu/Zn Superoxide dismutase | S. aurata CAI79044 | 5.4/7.0 | 66/44 | 2/2 | HVGDLGNVTAGADNVAK (4) MLTLSGPLSIIGR (0.14) |
H5 | Histone H2A | D. rerio | 10.6/13.5 | 49/7 | 1/1 | AGLQFPVGR (0.00014) |
H6 | 14–3-3 protein beta/alpha-1 | O. mykiss Q6UFZ9 | 4.6/27.7 | 143/15 | 2/5 | YLSEVASGDSK (2.6*10−8) YLSEVASGDSKK (0.35) NLLSVAYK (8.3/10−5) VISSIEQK (1.3) DSTLIMQLLR (1.5*10−5) |
H7 | Apolipoprotein A1 | S. aurata O42175 | 5.2/29.6 | 232/29 | 5/5 | AVLDVYLTQVK (0.02) AVNQLDDPQYAEFK (0.0032) IEEMYTQIK (0.00025) SSLAPQNEQLK (0.00099) TLLTPIYNDYK (0.0014) EVVQPYVQEYK (0.092) ITPLVEEIK (0.0024) |
H8 | Phosphatidylethanolamine-binding protein 1 | S. aurata FM145015 | 9.1/29.7 | 174/13 | 3/2 | LYDQLAGK (28) LYTLALTDPDAPSR (0.0019) YGSVEIDELGK (0.00074) |
H9 | Apolipoprotein A1 | S. aurata O42175 | 5.2/29.6 | 183/19 | 5/5 | IEEMYTQIK (1.2) SSLAPQNEQLK (3.5) TLLTPIYNDYK (0.14) EVVQPYVQEYK (0.42) ITPLVEEIK (0.87) |
H10 | Actin cytoplasmic 1 | Ctenopharyngodon idella P83751 | 5.3/42.1 | 53/7 | 3/3 | AGFAGDDAPR (0.085) DLTDYLMK (0.089) GYSFTTTAER (6*10−5) |
H11 | Natural killer enhancing factor 2 | Larimichthys crocea XP_010732927 | 5.9/21.8 | 278/26 | 6/2 | DYGVLKEDDGIAYR (0.22) EDDGIAYR (21) IPLVADLTK (1.3*10−5) GLFVIDDK (0.41) QITINDLPVGR (0.00085) LVQAFQHTDK (0.34) |
H12 | ADP-ribosylation factor 3 | Takifugu rubripes P61207 | 6.8/20.7 | 106/24 | 4/4 | ILMVGLDAAGK (4*10−7) MLAEDELR (3*10−5) DAVLLVFANK (0.056) QDLPNAMNAAEITDK (0.17) |
H13 | Natural killer enhancing factor 1 | Osmerus mordax ACO 09982 | 5.8/22.3 | 102/14 | 3/3 | LAPDFTAK (26) AVMPDGQFK (18) QITINDLPVGR (0.0028) |
H14 | Glutathione S-transferase 3 | S. aurata AFV39802 | 6.9/25.5 | 206/19 | 5/3 | FTGILGDFR (0.00069) MTEIPAVNR (0.1) TVMEVFDIK (2.2) YLPVFEK (11) AILNYIAEK (0.79) |
H15 | Triosephosphate isomerase A | S. aurata FG266106 | 8.7/28.8 | 203/18 | 5/4 | IIYGGSVTGATCK (0.3) NVSEAVANSVR (0.0059) KNVSEAVANSVR (1200) GAFTGEISPAMIK (4.9) FGVAAQNCYK (11) |
H16 | Triosephosphate isomerase B | D. rerio Q90XG0 | 6.5/27.1 | 76/12 | 3/3 | FFVGGNWK (0.065) GAFTGEISPAMIK (5.7*10−7) WVILGHSER (0.037) |
H17 | Triosephosphate isomerase B | D. rerio Q90XG0 | 6.5/27.1 | 131/23 | 5/5 | FFVGGNWK (6.1) GAFTGEISPAMIK (1.7*10−6) WVILGHSER (0.001) HVFGESDELIGQK (2.9*10−6) VVLAYEPVWAIGTGK (0.022) |
H18 | ATP synthase subunit beta | Cyprinus carpio Q9PTY0 | 5.1/55.3 | 317/28 | 10/10 | TIAMDGTEGLVR (0.0043) VLDTGAPIR (1.8*10−6) IPVGPETLGR (7.4*10−8) IMNVIGEPIDER (1.1*10−6) VVDLLAPYAK (3*10−5) IGLFGGAGVGK (6.8*10−6) TVLIMELINNVAK (0.022) VALVYGQMNEPPGAR (5.4*10−5) IPSAVGYQPTLATDMGTMQER (0.0006) AIAELGIYPAVDPLDSTSR (0.0045) |
H19 | Actin-related protein | T. rubripes O73723 | 5.6/47.9 | 33/8 | 3/3 | FSYVCPDLVK (0.062) DYEEIGPSICR (0.0066) EVGIPPEQSLETAK (0.14) |
H20 | Actin cytoplasmic 1 | Oreochromis mossambicus P68143 | 5.3/42.1 | 144/15 | 5/5 | AGFAGDDAPR (3.8*10−8) VAPEEHPVLLTEAPLNPK (0.0038) DLTDYLMK (0.024) GYSFTTTAER (1.5*10−5) EITALAPSTMK (0.066) |
H21 | Macrophage-capping protein | L. crocea XP_010735467 | 5.8/38.7 | 185/12 | 5/4 | TQVEILPQGK (0.022) MKTQVEILPQGK (0.45) MPELAESTPEEDSK (0.16) EIASLIR (10) EGGVESGFR (1.8) |
H22 | Citrate synthase | Katsuwonus pelamis Q6S9V7 | 8.5/52.4 | 95/8 | 4/4 | DVLSDLIPK (0.25) ALGFPLERPK (0.061) VVPGYGHAVLR (3.7*10−5) IVPNVLLEQGK (1.1*10−6) |
H23 | Heat shock cognate 71 kDa | Oryzias latipes Q9W6Y1 | 5.8/76.6 | 476/20 | 13/3 | NQVAMNPTNTVFDAK (1.8*10 −7 ) SFYPEEVSSMVLTK (1.2*10 −5 ) GQIHDIVLVGGSTR (0.0077) VEIIANDQGNR (5.8*10−6) MKEIAEAYLGK (7.2*10−5) EIAEAYLGK (0.02) DAGTISGLNVLR (3.6*10−5) IINEPTAAAIAYGLDKK (1*10−6) STAGDTHLGGEDFDNR (0.0014) ARFEELNADLFR (5.5*10−5) FEELNADLFR (7.6*10−7) LLQDFFNGK (9.2*10−6) NGLESYAFNMK (0.00053) |
H24 | Heat shock cognate 71 kDa | Ictalurus punctatus P47773 | 5.2/71.6 | 306/14 | 8/2 | TTPSYVAFTDSER (1.8*10 −6 ) FELTGIPPAPR (0.00019) VEIIANDQGNR (2.3*10−7) MKEIAEAYLGK (0.096) DAGTISGLNVLR (7.8*10−7) STAGDTHLGGEDFDNR (0.00012) FEELNADLFR (7.2*10−8) LLQDFFNGK (0.0026) |
H25 | Keratin type I | O. mykiss NP_001117848 | 5.2/51.9 | 521/14 | 9/0 | KLEAANAELELK (1.7*10−9) LEAANAELELK (0.00012) LAADDFR (0.0068) TKYENELAMR (0.041) QSVEADIAGLKR (43) SDLEMQIEGLK (9.2*10−5) NHEEELLAMR (1.6) TRLEMEIAEYR (0.18) LEMEIAEYR (0.029) |
H26 | Complement component 3 | S. aurata ADM13620 | 8.1/186.9 | 152/4 | 7/6 | TLYTPESTVLYR (18) DITYLILSR (0.87) VTGDPEATVGLVAVDK (62) SVPFIIIPMK (13) DSSLNDGIMR (21) VVPQGVLIK (11) EYVLPSFEVK (100) |
H27 | Gelsolin | S. aurata HS984154 | 6.0/31.6 | 548/45 | 9/7 | QPGLQVWR (0.035) GGVASGFQHVVTNDMSAK (13) GDSFILDLGK (0.059) LHMVEEGEEPK (25) AFTEALGPK (2.1) TAIAPSTPDDEKADISNK (0.00049) GALYMISDASGTMK (0.0044) VSSVAPSSPFK (0.0033) QAMLSPEECYILDNGVDK (1600) IENLDLKPVPK (54) |
H28 | Immunoglobulin M heavy chain | S. aurata AFN20639 | 6.1/51.2 | 50/2 | 1/1 | GFSPNSFQFK (0.039) |