Table 3 Information of primers used for qPCR study
From: Chronic wounds alter the proteome profile in skin mucus of farmed gilthead seabream
Gene names | Accession number | Amplicon size | Sequence (5′ → 3′) |
|---|---|---|---|
Immunoglobulin mu heavy chain | JQ811851 | 113 | F: CAACATGCCCAATTGATGAG R: GGCACGACACTCTAGCTTCC |
Complement component 3 | HM543456 | 106 | F: CGCTCTTCTTGCTCTGGTGA R: CTGAGTTGATCCGTAGCCCC |
Histone 2b | AM953480 | 174 | F: AGACGGTCAAAGCACCAAAG R: AGTTCATGATGCCCATAGCC |
Heat shock cognate 71 kDa | HS987272 | 124 | F: GCCATGAACCCAACCAACAC R: GGCGGGTGTTGTCATTGATG |
Superoxide dismutase | AJ937872 | 103 | F: TCACGGACAAGATGCTCACT R: TCCTCGTTGCCTCCTTTTCC |
Glutathione s-transferase | JQ308828 | 111 | F: AGCGCTACCTTCCAGTGTTC R: CCTCCAACATCAGGGTGCAT |
Gelsolin | HS984154 | 105 | F: GCCATCAGAGCAACAGAGGT R: CTCACTGCCACACCACTGAT |
Actin beta | AF316854 | 352 | F: GGCACCACACCTTCTACAATG R: GTGGTGGTGAAGCTGTAGCC |
Keratin 1 | FJ744592 | 105 | F: AGAGATCAATGACCTGCGGC R: CCCTCTGTGTCTGCCAATGT |
Elongation factor 1 alpha | AF184170 | 115 | F: TGTCATCAAGGCTGTTGAGC R: GCACACTTCTTGTTGCTGGA |
Ribosomal protein s18 | AM490061 | 109 | F: CGAAAGCATTTGCCAAGAAT R: AGTTGGCACCGTTTATGGTC |