Fig. 4

DNA methylation regulates the seesaw between H3K4me1 and H3K4me3. Surface of enrichment of H3K4me3 versus H34me1 within regulatory sites of (a) ESCs, (b) cortex, and (c) liver cells. Blue and red points represent regulatory sites with DNA methylation lower and higher than 50%, respectively. (d) Number of H3K4me1 and H3K27me3 peaks in WT and Mll1 KO MEF cells. (e) Distribution of the WT DNA methylation in genomic regions specifically enriched of H3K4me3 in WT or Cfp1 KO ESCs. (f) Scatter plot of the changes in H3K4me3 versus H3K4me1 enrichment (only for the H3K4me3/1 peaks with DNA methylation <50%) from WT to Cfp1 KO cells. Blue and red points represent H3K4me1 peaks specific to WT and Cfp1 KO cells, respectively. (g) Distribution of H3K4me3 changes for different H3K4me1 peaks (only for the H3K4me3/1 peaks with DNA methylation <75%) specific to WT or Cfp1 KO cells. (h) DNA methylation, H3K3me1 and H3K4me3 profiles of WT (blue tracks) and Cfp1 KO (red tracks) within several loci. Five genomic regions (I to V) approximately covering the gene promoters are indicated with green segments above charts. The y-axis represents the DNA methylation measured as the percentage of reads that support the methylated state of each CpG (estimated methylation level). For each histone mark track, the y-axis represents the normalized level of ChIP-seq signal over the genomic regions