Fig. 1

Ribosome profiling of Toxoplasma gondii. a The experimental design. Cyclohexamide was added to the flasks for ~10 min prior to collecting the medium containing extracellular parasites and the host-cell monolayer was syringe lysed to release intracellular parasites. Chemically fragmented mRNA and sucrose gradient fractionated monosomes were used to prepare RNA-seq and Ribo-seq libraries, respectively, in parallel. b P-site tracks, colour-coded per frame, were created for all annotated exons in RiboTaper. c Ribo-seq (red peaks) and RNA-seq (blue peaks) read pile-up, presented as Fragment per kilobase exon per million reads (FPKM), on a multi-exonic gene. Exons are shown as blue blocks while introns are represented by blue lines