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Fig. 3 | BMC Genomics

Fig. 3

From: Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii

Fig. 3

Translation regulation is predominant in Toxoplasma gondii. a Although most transcripts showed no differential regulation (grey dots n = 4332) between the intracellular and extracellular parasites, several dysregulated genes were significantly (Benjamini–Hochberg FDR ≤ 0.1) regulated at the level of mRNA abundance only (green dots, RNA-ONLY, n = 650), ribosome occupancy only (black dots, RIBO-ONLY; n = 1339), and at the level of both mRNA abundance and ribosome occupancy (red dots, RIBO + RNA, n = 899). Differences in translational efficiency in intracellular versus extracellular parasites were calculated in Ribodiff on transcripts with average RNA-seq DESeq2 normalized read counts ≥ 5. b The slope for red spots approached 1 (x = y), indicating equal fold changes in gene regulation at the level of mRNA abundance and ribosome occupancy between the intracellular and extracellular parasites (standardized major-axis estimation; SMA). Significant (P < 2.22e-16) divergence of black and red slopes demonstrate differences in gene regulation at the level of ribosome occupancy (RIBO-ONLY) and mRNA abundance (RNA-ONLY), respectively

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