Fig. 2

Re-assembly of barcoded sequence data from Lam et al... dataset. Assemblies of bar-coded sequence data from Lam et al. were used to evaluate results achieved by different tool and pipelines in the analysis of pooled sequencing data. Of the 92 clones subjected to individual sequencing by Lam et al. [16] showed lack of coverage or absence of end tags sequences and were therefore discarded from these analyses. Of the 61 inserts for which sequencing information for both “end-tags” was available (“complete”, Indicated in black), 46 were matched correctly to both ends (Fully assigned), 11 were matched with only 1 out of 2 ends (Partially assigned) and 2 did not show any similarity to available insert termini sequences. Of the twelve clones for which only “partial” (single end-tag, indicated in red) sequencing information is available ten were recognized and assigned with the proper end tag while 2 are missing. Only assigned and partially assigned contigs were included in the benchmark dataset used for the evaluation