Fig. 3

Alignments of GhNPCs. Multiple sequence alignments of GhNPCs were performed using the software MEGA7 and tinted using DNAman. Six motifs which were annotated as the phosphoesterase domain were marked in yellow. Putative active site (marked in blue) and conserved Asp-Arg ion pair (marked in purple) was indicated according to Pokotylo et al. [1]. Secondary structures of GhNPCs were predicted using JPred4 server [29]. Beta sheet were marked in green arrow and alpha helices were marked in red rectangle