Fig. 5

Phylogenetic tree of plant NPC. The tree was constructed using MEGA7 using the Maximum Likelihood method. Branches corresponding to partitions reproduced in less than 50% bootstrap replicates are collapsed. The bootstrap consensus tree inferred from 1000 replicates is taken to represent the evolutionary history of the taxa analyzed. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using a JTT model, and then selecting the topology with superior log likelihood value. The analysis involved 59 amino acid sequences. Species abbreviations: At, Arabidopsis thaliana; Gm, Glycine max; Os, Oryza sativa; Pp, Physcomitrella patens; Sb, Sorghum bicolor; Sm, Selaginella moellendorffii; Gh, Gossypium hirsutum; Ga, G. arboretum; Gr, G. raimondii; NPC, non-specific phospholipase C