Fig. 3

CBP is required for expression of Kr-h1 in Tribolium larvae. The newly emerged final instar larvae were injected with 1 μg of either dsmalE or dsCBP. Seventy-two hours later, 0.5 μl of 10 μM hydroprene or cyclohexane (control) was topically applied. Six hours after the application, total RNA was isolated and used to quantify mRNA levels of CBP (a) and Kr-h1 (b) in each treatment by qRT-PCR using RP49 as a control. The data shown are M + SE (n = 3). (Letters represent significance at 95% CI)