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Fig. 5 | BMC Genomics

Fig. 5

From: A prototypical non-malignant epithelial model to study genome dynamics and concurrently monitor micro-RNAs and proteins in situ during oncogene-induced senescence

Fig. 5

CDC6 induction resulted in R loop formation. a CDC6 induction increased total transcription levels, as measured by 5’-EU incorporation. Scale bar: 35 μm. b IF for S9.6, antibody specific for DNA:RNA hybrids, indicated increased possibility of R loop formation upon CDC6 expression. Concurrent IF detection of nucleolin, revealed nucleolus subcellular localization of the R loops. DNA:RNA hybrids disappeared after treatment with RNase A or RNase H showing the specificity of the reaction. Scale bar: 15 μm. c Double IF for 53BP1 and UBF showed re-localization of UBF from the nucleolar interior to the periphery associated with the perinucleolar distribution of 53BP1 foci (indicated by white arrows) reflecting DNA damage repair in heterochromatin-related structures [96]. Scale bar: 10 μm. d Heatmap of factors affecting R loops (Additional file 11: Table S4) [98]

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