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Fig. 8 | BMC Genomics

Fig. 8

From: A prototypical non-malignant epithelial model to study genome dynamics and concurrently monitor micro-RNAs and proteins in situ during oncogene-induced senescence

Fig. 8

In situ detection of miR34c in senescent cells. Detection of miR34c in senescent cells employing the HBEC CDC6 Tet-ON system. Double staining was performed in two consecutive states: in the OFF state where proliferation of HBECs is evident (“OFF”) and 6 days after constitutive induction of CDC6 when cells are senescent (“6d ON”). Step 1: Fluorescence FISH of miR34c employing a double-DIG-labeled LNA probe, visualized as green emission in the cytoplasm, using TSA plus Fluorescein (emitting at 518 nm). Step 2: GL13 staining, visualized at far red spectra as granules in the cytoplasm employing Alexa Fluor goat-anti-mouse (647 nm) (emitting at 668 nm). Scale bar: 50 μm

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