Fig. 2

Characteristics of the tTAV circuit. The crossing scheme and media used are described in detail in the Methods, but, briefly, the homozygous 102D–tTAV line was crossed to a ‘white eyed’ w1118 line. Red-eyed wildtype flies, indicating the presence of the tTAV-system, were back crossed to the w1118 line and the percentage of tTAV flies in the F2 generation on various types and concentrations of antibiotic media was determined. Due to the use of tTAV heterozygotes, 50% survival represents the maximum percentage of the offspring that can inherit tTAV. Each data point is the median of 10 replicates. The data are presented as a standard boxplot with whiskers extending to the lowest or highest value within 1.5 times the IQR from the hinge. a Median survivorship for 4 members of the tetracycline class of antibiotics across dosages spanning 5 orders of magnitude. b Higher dosage resolution of median survivability for tetracycline and doxycycline, showing critical thresholds for antibiotic rescue. The IC50 values for doxycycline and tetracycline are 0.161 and 3.338, respectively