Fig. 4

Validation of circRNA age-accumulation. a RNA-seq quantification of select circRNAs during aging (TPM fold-change with L4 set at 1). Total number of reads across all libraries for each circRNA is noted above graph. Labels display circRNA names with host gene in brackets. b RT-qPCR data for the same selected circRNAs as in A). Data is normalized to cdc-42 mRNA. Note the greater magnitude of age-related expression changes reported by RT-qPCR versus RNA-seq for all circRNAs. Note that for cel_circ_0001331, there was a significant reduction (P < 0.05) between D-1 and D-10 (Additional file 5: Table S3). Error bars represent SEM. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001, two-tailed t-test compared to L4