Fig. 1

Expression patterns of the GmAGL1 gene. a Gene expression of GmAGL1 in various tissues of the five homozygous transgenic lines and WT. The T2 generations of the two homozygous transgenic lines GmAGL1-OX lines 1 and 5 and WT were cultured in soil. RNA was extracted from roots, stems, leaves, and shoot apical meristems (SAMs) of 20-day-old seedlings. RNA was extracted from flowers and pods of 50-day-old seedlings. WT leaves were used as a control (relative expression of 1) to compare the relative expression of other samples. b Gene expression of GmAGL1 in the V0-R1 period in five homozygous transgenic plant SAMs. The T2 generations of the five homozygous transgenic lines, GmAGL1-OX lines 1 and 5, and WT were cultured in soil. RNA of SAMs was extracted from plants at the VC to R1 stages. VC (cotyledon) stage: single leaf half expanded, leaf margin separated; V1 (one-node) stage: single leaf fully grown, leaf margin of the first compound leaf separated; V2 (two-node) stage: the first compound leaf above the single leaf fully grown; V3 (three-node) stage: including the single leaf, three leaves on the main stem fully grown; V4 (four-node) stage: including the single leaf, four leaves on the main stem fully grown; R1 stage (the beginning of flowering): a flower is open at any position on the main stem. SAMs of WT-VC were used as a control (relative expression of 1) to compare relative expression in the other samples. GmAGL1 transcript levels were determined by qRT-PCR. The α-Tubulin gene was amplified as an internal control to normalize all the data. Transcript levels were calculated using the 2^-ΔΔCt formula to measure the expression levels relative to GmAGL1. Data are presented as the means of three biological replicates, and error bars indicate SDs. Asterisks indicate a significant difference between the WT and transgenic plants (**P < 0.01, *P < 0.05)