Table 1 Summary statistics of Illumina sequencing, assembly and annotation of normalized transcriptomes from three Japanese Ranidae species, including AMP transcripts identified
Species | Samples (No. of immune-related enriched GO terms) | No. of assembled contigs | Average contig length (bp) | No. annotated contigs | AMP gene families identified | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Â | Uniprot | Ensembl human | GO | Pfam | KO | Total | ||||
R. japonica | blood (2) skin (6a) spleen (46a) s29 tadpole body (2) | 312,172 | 650 | 54,984 (17.61%) | 49,635 (15.90%) | 52,377 (16.79%) | 36,646 (11.74%) | 42,592 (13.64%) | 57,339 (18.37%) | bradykinin, brevininb, histone, japonicinb, kassorin, kininogen, nigrosin, odorranain, pleurain, preprobrevininb, preprochensinin, ranacyclin, ranatensin, temporin |
R. ornativentris | blood (5) skin (7a) spleen (19a) s24 tadpole skin (3a) s29 tadpole body (3a) | 646,586 | 561 | 101,666 (15.72%) | 88,074 (13.62%) | 97,143 (15.02%) | 61,503 (9.51%) | 77,264 (11.95%) | 107,391 (16.61%) | andersonin, bombesin, bradykinin, brevinin, gaegurin, histone, japonicin, kininogen, nigroain, odorranainb, peptide DK25, pleurainb, preprobrevininb, preprochensininb, prepropalustrin, preproranatuerin, ranacyclin, ranatuerinb, tigerinin |
R. t. tagoi | blood (6a) skin (3) spleen (38) | 303,238 | 634 | 56,202 (18.54%) | 49,321 (16.26%) | 53,866 (17.76%) | 34,432 (11.35%) | 42,951 (14.16%) | 58,980 (19.45%) | andersonin, bradykininb, brevinin, daunchinain, esculentin, gaegurin, histone, japonicin, kininogen, lividin, preprobrevininb, preprochensinin, prepropalustrin, ranatuerin, temporinb |