Table 1 Bacterial strains, plasmid vectors, plasmid constructs and primers used in this study

Strains

Xc-03-1638-1-1

Red Blush Grapefruit (C. paradise Macf.), 2003, Bella Vista, Corrientes, Argentina. CuR

Xcc-03-1638-1-1

Xc-A306

Orange (C. sinensis L. Osbeck) 1997, Paranavai, Parana, Brazil. CuS

Rui Pereira Leite Jr. DPI [5]

Xc-A104

Eureka Lemon (C. limon L. Burm. f.), 2008, Bella Vista, Corrientes, Argentina. CuR

Xcc-08-3420

Xc-A79

Clemenule Tangerine (Citrus ×clementina), 2007, Monte Caseros, Entre Rios, Argentina. CuR

Xcc-07-3179

Xc-A86

Red Blush Grapefruit (C. paradisi Macf.) 2003, Bella Vista, Corrientes, Argentina. CuR

Xcc03–1638 (LM180) [18]

Xc-A2090

Lime (Citrus sp.), 2004, Monroe County, FL. Lack of pthA1 and pthA4.

DPI

Xc-A1660

Dooryard citrus tree, 2004, Palm Beach County, FL. Idem Xc-2090 DPI

DPI

Xc-AEtrog

Dooryard citrus tree, 2003, Orange County, FL.

DPI

Xc-A100

Satsuma Okitsu (C. unshiu Marc.) 1993, Japan). Weak pathogenicity only in KL. No pathogenicity reaction in GF. StrpR

Xcc-93-1900

Xc-A109

Key lime (C. aurantifolia (Christm.) Swingle), 2002, Madras, India. Normal pathogenicity in KL, no pathogenic in GF

Xcc-02-1888

Xv1111

Tomato, 1955, New Zealand. CuR

ATCC 35937.

Xv BV5-4a

Tomato, 1987, Bella Vista, Argentina. CuR

Canteros, B. I.

Xp 1–7

Tomato, 2006, Florida. CuR

Stall, R. E.

SW2

Corn, 1974, Ohio. Erwinia stewartii SW2, 13 mass characterized plasmids.

[42]

Plasmids

pGMTE

pGEMT-easy, AmpR

Promega Corp. (Madison, WI, USA)

pBluescript II SK(+)

Phagemid, pUC derivative, AmpR

Stratagene (La Jolla, CA, USA)

pLAFR3

Tra − Mob+, RK2 replicon, tetR

[45]

Primers

SPCF33–1-F

5’-GCACGTTCTTCTTGGAAGCA-3’

This study

SPCF33–1-R

5’-CCTGGATGAAGTAGTGCAAT-3’

SPCF64–1-F

5’-CACTCAACGAGTCCCAGCTT-3’

This study

SPCF64–1-R

5’-GTAATCAGGGCGTGCAGGCG-3’

J-pthA1

5′- CTTCAACTCAAACGCCGGAC-3’

[23]

J-pthA2

5′- CATCGCGCTGTTCGGGAG-3′