Fig. 9

Analysis of the cell wall biosynthetic pathway on the transcript, protein and metabolite levels. The abundance of the secondary cell wall-related metabolites, transcripts (Trans) and proteins (Prot) are shown in the PttSCAMP3 RNAi lines compared to the wild type. ↑ in the blue boxes indicates upregulation in PttSCAMP3 RNAi lines; ↑↑ in the blue boxes indicates significant (|p(CORR)| >0.5) upregulation in PttSCAMP3 RNAi lines; ↓ in the red boxes indicates downregulation in the PttSCAMP3 RNAi lines; ↓↓ in the red boxes indicates significant (|p(CORR)| > 0.5) downregulation in PttSCAMP3 RNAi lines. Metabolites are shown in bold in boxes with the arrow/color codes as indicated above. The genes and proteins correspond to the following Populus trichocarpa gene models according to JGI V3.0: Fructokinase 1 (Potri.017G126300); Fructokinase 2 (Potri.007G129700); Fructokinase 3 (Potri.017G029000); Fructokinase 4 (Potri.012G132700); Fructokinase 5 (Potri.004G089300); Fructokinase 6 (Potri.015G134900); Fructokinase 7 (Potri.019G063600); Hexokinase 1 (Potri.001G190400); Hexokinase 2 (Potri.005G238600); Hexokinase 3 (Potri.009G050000); Hexokinase 4 (Potri.018G088300); Hexokinase 5 (Potri.001G254800); Cytosolic INV 1 (Potri.014G188100); Cytosolic INV 2 (Potri.013G110800); Cytosolic INV3 (Potri.019G082000); sucrose synthase 3 (Potri.002G202300); sucrose synthase 4 (Potri.006G136700); sucrose synthase 5 (Potri.018G063500); sucrose synthase 6 (Potri.004G081300); sucrose synthase 7 (Potri.012G037200); glucose-6-phosphate isomerase 1 (Potri.008G118900); glucose-6-phosphate isomerase 2 (Potri.002G104000); UDP-D-apiose/UDP-D-xylose synthase 1 (Potri.009G150600); UDP-D-apiose/UDP-D-xylose synthase 2 (Potri.004G189900); UDP-glucose 6-dehydrogenase 1 (Potri.017G092000); UDP-glucose 6-dehydrogenase 2 (Potri.004G118600); UDP-glucose 6-dehydrogenase 3 (Potri.010G159800); UDP-glucose 6-dehydrogenase 4 (Potri.008G094300); UDP-glucose 4-epimerase 1 (Potri.003G123700); UDP-glucose 4-epimerase 2 (Potri.003G140900); UDP-glucose 4-epimerase 5 (Potri.001G090700); UDP-D-xylose synthase (UXS) 1 (Potri.006G214000); UDP-D-xylose synthase (UXS) 2 (Potri.014G129200); UDP-D-xylose synthase (UXS) 3 (Potri.010G207200); UDP-D-xylose synthase (UXS) 4 (Potri.002G204400); UDP-D-xylose synthase (UXS) 5 (Potri.001G237200); UDP-D-xylose synthase (UXS) 6 (Potri.008G053100); UDP-D-xylose synthase (UXS) 7 (Potri.016G080500); Transketolase 1 (Potri.002G146300); Transketolase 2 (Potri.014G068200); UDP-D-glucuronate 4-epimerase (Potri.002G146500); Rhamnose biosynthesis 1 (Potri.006G272700); Rhamnose biosynthesis 2 (Potri.001G383500). Observe that the numbering of the genes is arbitrary to allow identification of the different members of the gene family