Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure

BMC Genomics

Table 2 Genes with ≥20 fold-change in at least two time points for PMA exposure (n = 17)

Gene ID	Gene Name	Product Description	Hg t10	Hg t30	Hg t60	PMA t10	PMA t30	PMA t60
b3728	pstS	ABC phosphate transport system; periplasmic binding protein	n.s.	16	8	86	165	n.s.
b1020	phoH	ATP-binding protein, function unknown	8	71	221	61	365	n.s.
b0996	torC*	c-Type cytochrome	n.s.	n.s.	n.s.	55	65	77
b4060	yjcB	Function unknown	33	11	12	52	35	n.s.
b0399	phoB	Positive response regulator for pho regulon	4	22	3	51	105	n.s.
b1530	marR	Transcription repressor of multiple antibiotic resistance	26	7	n.s.	48	29	n.s.
b1531	marA	Transcriptional activator for multiple antibiotic resistance;	15	9	n.s.	46	31	n.s.
b1112	bhsA	Biofilm, cell surface and signaling defects, YhcN family	1949	563	94	46	21	n.s.
b1532	marB	marRAB multiple antibiotic resistance operon	16	8	n.s.	45	27	n.s.
b4663	azuC	Function unknown; membrane-associated	27	25	36	41	49	n.s.
b4030	psiE	Pho regulon, regulated by phoB and cAMP	35	64	17	38	59	n.s.
b3686	ibpB	Chaperone, heat-inducible protein of HSP20 family	402	124	281	37	211	9
b1748	astC	Succinylornithine transaminase; carbon starvation protein	30	31	25	34	36	n.s.
b3469	zntA	Zn(II), Cd(II), and Pb(II) translocating P-type ATPase	40	19	8	29	22	n.s.
b2582	trxC	Thioredoxin 2, zinc-binding; Trx2	134	50	7	24	24	n.s.
b4354	yjiY	Predicted transporter, function unknown	n.s.	n.s.	n.s.	20	77	n.s.
b1625	cnu	OriC-binding complex H-NS/Cnu	n.s.	10	n.s.	20	24	n.s.

Table is sorted by PMA at 10 min column. n.s. = not significantly different from unexposed culture and boldface highlights actual values ≥20-fold. Gene names in boldface have a ≥ 20 differential expression response to both Hg and PMA in at least one time point (n = 11). Genes marked with an asterisk (*) had fewer than 11 average read counts for at least 2 time points in the unexposed condition and were operationally defined as being “off” in that condition. Such genes constituted, the lowest 15th percentile of all genes, based on raw read counts normalized across all libraries and across all biological replicates

ISSN: 1471-2164