Table 1 Alignment of WES data from the human-mouse DNA dilution series
% of human DNA | %of mouse DNA | Replicate | Alignment efficiency human genome % | Alignment efficiency combined genome (%) | %of reads mapped on the human genome | %of reads mapped on the mouse genome | Estimated human DNA content |
|---|---|---|---|---|---|---|---|
100 | 0 | a | 90.81 | 90.64 | 99.98 | 0.02 | 99.89 |
100 | 0 | b | 90.38 | 90.16 | 99.98 | 0.02 | 99.88 |
100 | 0 | c | 90.26 | 90.13 | 99.98 | 0.02 | 99.89 |
90 | 10 | a | 88.57 | 90.43 | 97.59 | 2.41 | 90.22 |
90 | 10 | b | 88.53 | 90.39 | 97.58 | 2.42 | 90.16 |
50 | 50 | a | 75.65 | 89.37 | 83.14 | 16.86 | 50.73 |
50 | 50 | b | 74.90 | 89.71 | 82.18 | 17.82 | 49.01 |
50 | 50 | c | 75.41 | 89.55 | 82.78 | 17.22 | 50.07 |
25 | 75 | a | 58.07 | 89.25 | 62.44 | 37.56 | 23.97 |
25 | 75 | b | 60.25 | 89.48 | 64.58 | 35.42 | 26.17 |
0 | 100 | a | 7.01 | 88.77 | 0.10 | 99.90 | 0.00 |
0 | 100 | b | 7.17 | 88.56 | 0.10 | 99.90 | 0.00 |
0 | 100 | c | 6.65 | 88.95 | 0.08 | 99.92 | 0.00 |