Fig. 5

Differential expression of nonclassical Dscam genes. a Heatmap of expression of 31 Dscam genes in various tissues of M. martensii. The expression level for each transcript is shown as reads per kilobase of transcript per million mapped reads (RPKM) of its corresponding constitutive exons. The 25-nt fragmented RNA-sequencing datasets were mapped to calculate the relative expression level. The maximum expression levels of mDscam8 were found in hemocytes, whereas mDscam10 and mDscam15 were highly expressed in poison glands. b The differing expression patterns of Dscams in I. scapularis lineages. This result indicates that many Dscams were preferentially and specifically expressed in certain lines. Data are expressed as the mean ± SD from three independent experiments