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Fig. 3 | BMC Genomics

Fig. 3

From: Unique, dual-indexed sequencing adapters with UMIs effectively eliminate index cross-talk and significantly improve sensitivity of massively parallel sequencing

Fig. 3

Level of cross-talk using combinatorial indices on the Illumina MiSeq and 2500 platforms. The 96-well plate layout represents the actual adapter plate arrangement. Numbers in each well represent the number of fragments that passed standard Illumina filters and demultiplexed using only perfect index matches on all TS-96 indices. a Four cell-line libraries were prepared using Illumina-synthesized TS-96 adapters (green), libraries were pooled, hybrid captured using a custom bait set, and then sequenced on an Illumina MiSeq v2 flow cell. b Fifteen patient-derived xenograft (PDX) libraries were prepared using IDT-synthesized TS-96 adapters (green), libraries were pooled, hybrid captured using a custom bait set, and then sequenced on a single lane of an Illumina HiSeq2500 flow cell

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