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Fig. 4 | BMC Genomics

Fig. 4

From: Unique, dual-indexed sequencing adapters with UMIs effectively eliminate index cross-talk and significantly improve sensitivity of massively parallel sequencing

Fig. 4

Level of cross-talk using unique, dual-matched indexed adapters on Illumina HiSeq 2500. The 96-well plate layout represents the adapter plate. A total of 35 adapters were synthesized. Seventeen human cell line libraries were prepared using IDT-synthesized unique, dual-matched indexed adapters (green), libraries were pooled, hybrid captured using a custom bait set, and then sequenced on a single lane of an Illumina HiSeq2500 flow cell. Numbers in each well represent the number of fragments that passed standard Illumina filters and demultiplexed using only perfect sequence matches on all TS-96 indices

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BMC Genomics

ISSN: 1471-2164

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