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Fig. 5 | BMC Genomics

Fig. 5

From: Unique, dual-indexed sequencing adapters with UMIs effectively eliminate index cross-talk and significantly improve sensitivity of massively parallel sequencing

Fig. 5

Unique dual-matched indices accurately identify contamination and index hopping events. a Heatmap displays the percent of reads for all i5 and i7 sample index combinations in 1-, 4-, 8- or 16-plex captures. The contamination level of library adapters was 0.09% for single library captures but increased up to 0.39% for 16-plex captures. b Percentage of reads filtered out per multiplex experiment when using dual-matched indexed adapters that would have been misassigned using combinatorial indices

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