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Fig. 6 | BMC Genomics

Fig. 6

From: Unique, dual-indexed sequencing adapters with UMIs effectively eliminate index cross-talk and significantly improve sensitivity of massively parallel sequencing

Fig. 6

UMI consensus calling improves variant detection accuracy, allows for the detection of rare variants, and corrects 8-oxoguanine errors. a Sensitivity and positive predictive value (PPV) using either no UMI or consensus calling for expected allele frequencies from 0.5–99.5% across 291 SNPs. Variant calling performed using VarDict with a threshold of 0.2%. b Variant calling thresholds (AF) with no UMI or consensus calling further improved sensitivity and PPV for low frequency variants (N = 54). There were 10 and 44 sites expected at 1% and 0.5% allele frequencies, respectively. c Sensitivity and PPV for low frequency variants expected at AF 0.5–1% when using no UMI versus consensus calling using a variant calling threshold of 0.2% with VarDict. d Number of false positive calls with or without UMI consensus using a variant calling threshold of 0.2%

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