Comparison of three assembly strategies for a heterozygous seedless grapevine genome assembly

BMC Genomics

Table 3 BAC and EST validation information for all four assemblies

Details		AP	PLAT_d	MERGE	PLAT*_GC
BAC sequences: 120,148 BAC bp 66,371,065	Top-hits-length (bp)	55,484,200	59,330,132	59,387,356	59,520,643
	Number of BAC sequences	108,172	111,600	111,649	112,325
	Top-hits-length with 90% identity and 90% coverage (bp)	46,565,227	53,082,062	52,959,509	52,980,593
	Number of BAC sequences	83,879	95,375	95,147	95,152
EST sequences: 511,685 EST bp: 320,415,647	Top-hits-length (bp)	264,199,117	265,484,201	267,151,205	268,356,483
	Number of EST sequences	463,419	466,569	467,024	467,548
	Top-hits-length with 90% identity and 70% coverage (bp)	237,629,769	237,868,274	241,453,791	244,324,283
	Number of EST sequences	393,826	395,439	400,650	405,329

One-to-one relations between each BAC, EST and scaffolds were constructed according to the longest alignment for each BAC and EST and the total of those alignment lengths (top-hits-length) was calculated

ISSN: 1471-2164