Fig. 2

Analysis of miR-427 compared to other miRNAs across all tissue types shows miR-427 is the primary miRNA expressed. a miR-427 constitutes 60–75% of all miRNA reads in all four tissue types with miR-428 the second most prevalent miRNA in NC, neural and ectoderm only b Five isoforms of miR-427 were expressed all of which showed a similar hairpin distribution over the four tissue types with isoform C the most abundant c Alignments of the five isoforms of Xenopus miR-427 and Zebrafish miR-430 hairpins. The mature 5′ and 3′ sequences are indicated with red boxes. The 3′ strand has strong conservation whilst the 5′ strand does not d Abundance plots show the isoforms of miR-427 are largely derived from changes in the 5′ end of the miRNA except in the blastula where a miR-427 isoform is generated due to changes in the 3′ end of the miRNA