Fig. 3
From: In-depth proteomic analysis of boar spermatozoa through shotgun and gel-based methods
Immunofluorescence quantification through flow cytometry. Columns labeled as “No” or “FITC” correspond to spermatozoa incubated without any antibodies or with FITC-conjugated secondary antibody only, respectively. a Percentage of immunopositive sperm cells and b Relative fluorescence intensity of labeled spermatozoa. Specific targeting is revealed above the blue line, suggesting for example for GLUT-5 detection, a lower proportion of cells being targeted (a) and which cells also show lowest or no specific detection of GLUT-5 (b)