Fig. 4
From: In-depth proteomic analysis of boar spermatozoa through shotgun and gel-based methods
Protein and mRNA transcript comparison for selected proteins. a Table summarizes: protein detection with westernblotting (WB), immunofluorescence (IF), or altogether with shotgun approach (Both) and relative intensities of PCR amplicon bands classified as strong (+++), mid (++), weak (+), and low (+/−). b Representative gel electrophoreses of AQP11 and SPMI (AQN-3) of six boars (B1-B6) are shown. NC = Negative control and Ladder with band size starting from 100 bp (100 bp increment). On the SPMI gel, the arrow-head indicates the position of the expected amplicon size while the second band correspond to primer dimers