Fig. 4
From: Glutamate synthases from conifers: gene structure and phylogenetic studies
Southern blot analysis to determine gene copy number. a A Southern hybridization was performed on 10 μg of P. pinaster DNA digested with three restriction enzymes. Lane 1: BamHI, Lane 2: SalI and Lane 3: HindIII were hybridized to a 32P–labelled probe of the Fd-GOGAT gene. b Southern blot analysis to determine the copy number of the NADH-GOGAT gene in pine. In total, 10 μg of P. pinaster DNA was digested with three restriction enzymes. Lane 1: BamHI, Lane 2: SalI and Lane 3: HindIII were hybridized to a 32P–labelled probe of the NADH-GOGAT gene. Numbers on the left correspond to DNA molecular markers in kb