Fig. 5

Distance from terminal loop to branchpoint (a) and to 3′ pre-miRNA end (b). Distance between branchpoint and Dicer cleavage site of 3′ miRNA (c). Note that the 3′ miRNAs are mainly located near the 3′ splice site. The two separate points on the left figure show the branchpoint frequencies of the 5′ pre-miRNA strand (green) and of the terminal loop (red) as a whole. The blue curve displays the branchpoint distribution along the 3′ pre-miRNA strand. On the center figure shown are the distances of two mirtron groups: with branchpoint within the 3′ pre-miRNA strand (blue) and with branchpoint within the terminal loop (red). On the right figure considered are the distances between the 5′ end of the 3′ miRNA and the branchpoint within the 3′ pre-miRNA strand. Negative values correspond to the branchpoints into miRNA sequence