Skip to main content
Fig. 2 | BMC Genomics

Fig. 2

From: Candidate SNP markers of reproductive potential are predicted by a significant change in the affinity of TATA-binding protein for human gene promoters

Fig. 2

Experimental verification of the selected candidate SNP markers by an electrophoretic mobility shift assay (EMSA) in vitro. Legend: a and b Examples of electropherograms in the case of ancestral (panel A: norm, wild-type, wt) and minor (panel b: minor) alleles of the candidate SNP marker rs33981098 within the human HBB gene promoter and the corresponding diagrams of experimental values. c The significant correlations between the ratio of KD values of the equilibrium dissociation constant of the TBP–ODN complex, which were either measured in vitro (Y-axis) or in silico predicted (X-axis). Solid and dashed lines or curves denote the linear regression and boundaries of its 95% confidence interval, calculated using software Statistica (StatsoftTM, USA). Circles denote the ancestral and minor alleles of the candidate SNP markers rs563763767, rs33981098, rs35518301, rs1143627, rs72661131, rs1800202, and rs7277748 being verified; r, R, τ, γ, and α are linear correlation, Spearman’s rank correlation, Kendall’s rank correlation, Goodman–Kruskal generalized correlation, and their significance, respectively.

Back to article page