Fig. 4

a Levels of VEGF decreased with age in the hippocampus of OXYS and Wistar rats with more pronounced decrease in OXYS rats. d. – days; mo. – months. The data are presented as mean ± SEM. ^ - differences with previous age, p < 0.05; * - differences with age-matched Wistar rats, p < 0.05. b Immunolabeling for VEGF (red) showed age-related decrease of the number of VEGF-positive vessels in the hippocampus of OXYS and Wistar rats with more pronounced changes in OXYS rats. Immunolabeling for amyloid-β (green) revealed its accumulation with age in the hippocampal vessels of OXYS rats. Borders of VEGF-positive vessels are shown. Asterisks indicate lumens of VEGF-positive capillaries. Arrows indicate amyloid-β content. The DAPI (blue) staining shows cell nuclei. The scale bar is 50 μm