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Table 1 Predicted cis-regulatory elements in the 5’-UTRs. The predictions were obtained by using Infernal 1.1.2 with the Rfam 12.1 database and compared with the primary 5′-end data set. The list is sorted by Bit Score in descending order. Abbreviation: n.a., not applicable

From: Transcriptome sequencing of the human pathogen Corynebacterium diphtheriae NCTC 13129 provides detailed insights into its transcriptional landscape and into DtxR-mediated transcriptional regulation

Infernal prediction with Rfam

RNA-Seq detection

Name

ID

Start

End

Bit Score

Strand

Status

Start

End

Gene

Cobalamin riboswitch

RF00174

1,066,117

1,066,317

90.8

+

observed

1,066,117

1,066,317

DIP1084

Cobalamin riboswitch

RF00174

862,214

862,414

81.8

+

not observeda

n.a.

n.a.

n.a.

cspA

RF01766

285,506

285,923

64.8

+

observed

285,506

285,923

DIP0320 / cspA

mraW

RF01746

1,640,095

1,639,987

70.2

–

observedb

1,640,095

1,639,987

DIP1606 / mraW

TPP riboswitch

RF00059

924,778

924,890

60.8

+

observedc

924,778

924,890

DIP0953

TPP riboswitch

RF00059

922,838

922,969

60.0

+

observedc

922,838

922,969

DIP0951

msiK

RF01747

509,334

509,277

48.2

–

observed

509,334

509,277

DIP0539

TPP riboswitch

RF00059

27,456

27,566

57.6

+

not observeda

n.a.

n.a.

n.a.

  1. aNo TSS detected and insufficient read coverage in that area
  2. bMotif of the mraW region is located upstream of the TSS of DIP1606 / mraW
  3. cLast base of TPP riboswitch is located in the respective CDS