Fig. 4

FuSpot Visualization of ACACA-STAC2 True Fusion Gene Using FusionMap Supporting Reads. FuSpot’s visualization of the lab-validated ACACA-STAC2 fusion gene from Edgren et al. [22] The breakpoint for the fusion is marked by the dotted line in the center of the plot. Each colored horizontal line in the plot corresponds to one read local to the fusion breakpoint that was realigned by FuSpot. On either side of the breakpoint, references used for realignment are assigned a unique color in the legend atop the plot and the reads that align to each reference will take on the same color. Each read seen here was reported by the FusionMap fusion detector tool when run on the data set from Edgren et al. [22] As supporting read evidence, FusionMap provides exclusively single-end reads which align over the breakpoint. The even distribution of these reads about the breakpoint support the existence of the candidate fusion, but the omission of the mates leaves the picture incomplete; specifically, it excludes any reads which, along with their paired mates, may align on either side of the breakpoint