Fig. 6

FuSpot Visualization of ACACA-STAC2 True Fusion Gene Using Extracted STAR Supporting Reads. FuSpot’s final visualization of the lab-validated ACACA-STAC2 fusion gene from Edgren et al. [22] Each read seen here was extracted from either the normal or chimeric output file produced by aligning the data set from Edgren et al. [22] with STAR. All reads were extracted using the FuSpot extraction tool available on the FuSpot website. In the plot, these reads make up 4 distinct groups. Group 1 is made up of chimeric reads which represent a clear expression of the putative fusion gene. Groups 2 and 3 contain reads extracted from STAR’s normal alignment file which support the normal ACACA and normal STAC2 transcripts respectively. Group 4 is made up of reads that were extracted from the normal alignment file, but that are truly chimeric reads. These four groups exemplify the utility of FuSpot in building a comprehensive visual representation of a putative fusion