|
Target gene
|
Gene function
|
Primer sequences (5’-3’)
|
Amplification efficiencya
|
Tm (°C)
|
|---|
|
DELLA
|
SAR signalling
|
F: GTCTTCTAATTCAAACCA
|
1.926 ± 0.027
|
53
|
| | |
R: ATATCTGTTTACCCAAGTAA
| | |
|
RBP-hnRNP
|
Transcriptional factor
|
F: GAGAAAGATATTTGTTGGAG
|
1.947 ± 0.024
|
51
|
| | |
R: TGATCGTACATTACTACAACA
| | |
|
PGIP
|
Anti fungal compound
|
F: TGAAGGTGATGCTTCTATGCT
|
2.013 ± 0.026
|
53
|
| | |
R: GACTCACATCCAACGTTGCT
| | |
|
PR2
|
Anti fungal compound
|
F: GGCATGCTGGGAAACAATCT
|
2.018 ± 0.023
|
52
|
| | |
R: TGGCACACCTAACATGAGCT
| | |
|
PR10
|
Anti fungal compound
|
F: TGGCACTTCTGCTGTTAGATGGAC
|
2.017 ± 0.083
|
50
|
| | |
R: GGTAATCCATCCAGCCATTTGGAG
| | |
|
PP2A
|
Reference gene
|
F: GCCTCATTTGCAGCTGGTTT
|
2.002 ± 0.025
|
53
|
| | |
R: TACTCTCGTTCTAGGGTCCT
| | |
- aMean and standard deviation of the amplification efficiency for each gene were calculated from the coefficients of linear regression models fitted to each reaction (value of 2 equivalents to 100% efficiency, see [43])
