Transcriptome profiling of lentil (Lens culinaris) through the first 24 hours of Ascochyta lentis infection reveals key defence response genes

Table 2 Genes and primers used in qRT-PCR assay

Target gene	Gene function	Primer sequences (5’-3’)	Amplification efficiency^a	Tm (°C)
DELLA	SAR signalling	F: GTCTTCTAATTCAAACCA	1.926 ± 0.027	53
		R: ATATCTGTTTACCCAAGTAA
RBP-hnRNP	Transcriptional factor	F: GAGAAAGATATTTGTTGGAG	1.947 ± 0.024	51
		R: TGATCGTACATTACTACAACA
PGIP	Anti fungal compound	F: TGAAGGTGATGCTTCTATGCT	2.013 ± 0.026	53
		R: GACTCACATCCAACGTTGCT
PR2	Anti fungal compound	F: GGCATGCTGGGAAACAATCT	2.018 ± 0.023	52
		R: TGGCACACCTAACATGAGCT
PR10	Anti fungal compound	F: TGGCACTTCTGCTGTTAGATGGAC	2.017 ± 0.083	50
		R: GGTAATCCATCCAGCCATTTGGAG
PP2A	Reference gene	F: GCCTCATTTGCAGCTGGTTT	2.002 ± 0.025	53
		R: TACTCTCGTTCTAGGGTCCT

^aMean and standard deviation of the amplification efficiency for each gene were calculated from the coefficients of linear regression models fitted to each reaction (value of 2 equivalents to 100% efficiency, see [43])

ISSN: 1471-2164