Fig. 5
From: Systematic comparison of small RNA library preparation protocols for next-generation sequencing
The differences in detection sensitivity among protocols do not change at increased sequencing depth. Sequencing libraries were prepared using three TruSeq protocols (TS1, TS5 and TS7), two NEXTflex protocols (Nf1- and 6), and the SMARTer protocol (S) with human (a) or Arabidopsis (b) sRNA. A total of 20 million sequences were generated for each library. For each protocol, 0.1, 0.5, 1, 2, 5, 10, 15 or 20 million reads were trimmed, the reads with 19–24 nt inserts were mapped to miRBase and we calculated the number of identified miRNAs