Table 1 Overview of the protocols used in this study
From: Systematic comparison of small RNA library preparation protocols for next-generation sequencing
kit used | protocol | modifications |
|---|---|---|
Illumina TruSeq | TS1 | none: standard conditions |
TS2 | HD adapters used instead of Illumina adapters | |
TS3 | 3′ adapter ligation 16 °C o/n in the presence of PEG | |
TS4 | HD adapters used instead of Illumina adapters | |
3′ adapter ligation 16 °C o/n in the presence of PEG | ||
TS5 | HD adapters used instead of Illumina adapters | |
3′ adapter ligation 16 °C o/n in the presence of PEG | ||
purification step after 3′ adapter ligation | ||
TS6 | MRL adapters used instead of Illumina adapters | |
TS7 | MRL adapters used instead of Illumina adapters | |
3′ adapter ligation 16 °C o/n in the presence of PEG | ||
purification step after 3′ adapter ligation | ||
BIOO Scientific NEXTflex V2 | Nf1 | none: standard conditions |
Nf2 | 3′ adapter ligation 16 °C o/n | |
Nf3 | Illumina adapters instead of BIOO Scientific (HD) adapters | |
3′ adapter ligation 16 °C o/n | ||
Nf4 | no PEG in the ligation reactions | |
Nf5 | MRL adapters used instead of BIOO Scientific adapters | |
no PEG in the ligation reactions | ||
Nf6 | MRL adapters used instead of BIOO Scientific adapters | |
3′ adapter ligation 16 °C o/n | ||
New England Biolabs NEBNext | NN | none: standard conditions |
Clontech SMARTer | S | none: standard conditions |
Diagenode CATS kit | C | none: standard conditions |