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Table 1 Overview of the protocols used in this study

From: Systematic comparison of small RNA library preparation protocols for next-generation sequencing

kit used protocol modifications
Illumina TruSeq TS1 none: standard conditions
TS2 HD adapters used instead of Illumina adapters
TS3 3′ adapter ligation 16 °C o/n in the presence of PEG
TS4 HD adapters used instead of Illumina adapters
3′ adapter ligation 16 °C o/n in the presence of PEG
TS5 HD adapters used instead of Illumina adapters
3′ adapter ligation 16 °C o/n in the presence of PEG
purification step after 3′ adapter ligation
TS6 MRL adapters used instead of Illumina adapters
TS7 MRL adapters used instead of Illumina adapters
3′ adapter ligation 16 °C o/n in the presence of PEG
purification step after 3′ adapter ligation
BIOO Scientific NEXTflex V2 Nf1 none: standard conditions
Nf2 3′ adapter ligation 16 °C o/n
Nf3 Illumina adapters instead of BIOO Scientific (HD) adapters
3′ adapter ligation 16 °C o/n
Nf4 no PEG in the ligation reactions
Nf5 MRL adapters used instead of BIOO Scientific adapters
no PEG in the ligation reactions
Nf6 MRL adapters used instead of BIOO Scientific adapters
3′ adapter ligation 16 °C o/n
New England Biolabs NEBNext NN none: standard conditions
Clontech SMARTer S none: standard conditions
Diagenode CATS kit C none: standard conditions
  1. The Illumina TruSeq (‘TS’), BIOO Scientific NEXTflex V2 (‘Nf’) or Clontech SMARTer (‘S’) kits were used following their respective standard protocols or variants thereof. The different variants of the protocols are distinguished by numbers and their respective modifications are indicated