Table 1 miRNA primers used for validation of microarray results (Exiqon, Denmark)
miRNA Primer | Target sequence | Accession (Exiqon) |
|---|---|---|
hsa-miR-1 LNA™ PCR primer set, UniRT | UGGAAUGUAAAGAAGUAUGUAU | MIMAT0000416 |
bta-miR-9-5p LNA™ PCR primer set, UniRT | UCUUUGGUUAUCUAGCUGUAUG | MIMAT0009389 |
hsa-miR-128-3p LNA™ PCR primer set, UniRT | UCACAGUGAACCGGUCUCUUU | MIMAT0000424 |
hsa-miR-133a-3p LNA™ PCR primer set, UniRT | UUUGGUCCCCUUCAACCAGCUG | MIMAT0000427 |
hsa-miR-139-5p LNA™ PCR primer set, UniRT | UCUACAGUGCACGUGUCUCCAGU | MIMAT0000250 |
hsa-miR-145-5p LNA™ PCR primer set, UniRT | GUCCAGUUUUCCCAGGAAUCCCU | MIMAT0000437 |
hsa-miR-206 LNA™ PCR primer set, UniRT | UGGAAUGUAAGGAAGUGUGUGG | MIMAT0000462 |
hsa-miR-486-5p LNA™ PCR primer set, UniRT | UCCUGUACUGAGCUGCCCCGAG | MIMAT0002177 |
cfa-miR-503 LNA™ PCR primer set, UniRT | UAGCAGCGGGAACAGUACUG | MIMAT0006746 |
bta-miR-660 LNA™ PCR primer set, UniRT | UACCCAUUGCAUAUCGGAGCUG | MIMAT0004344 |
U6 snRNA LNA™ PCR primer set, UniRT (reference RNA) | GUGCUCGCUUCGGCAGCACAUAU ACUAAAAUUGGAACGAUACAGAG AAGAUUAGCAUGGCCCCUGCGCA AGGAUGACACGCAAAUUCGUGAA GCGUUCCAUAUUUUU | without MIMAT # |