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Fig. 4 | BMC Genomics

Fig. 4

From: Summarizing performance for genome scale measurement of miRNA: reference samples and metrics

Fig. 4

Observed distribution of log2 ratios. Panels A to F correspond to measurement processes A to F described in Results. For each datapoint in panels A to F, the difference between the Mix1 and Mix2 log2 signals (log2 ratios) is plotted against their average for each detected miRNA. Filled circles correspond to observed values for tissue-selective miRNA (those miRNA that are at least 10 times more prevalent in one pure total RNA tissue type relative to the other two) or miRNA that were approximately equal in relative abundance between placenta and brain (1-to-1): red = placenta-selective, blue = brain-selective, and yellow = 1-to-1 (liver-selective and placenta = brain). Open circles correspond to detectable, but non-selective miRNA. Red, yellow, and blue transparent bands indicate the 95% confidence interval for the loess (locally weighted smoothing) function (black lines) for the placenta, 1-to-1, and brain subsets, respectively. Panel F includes data from three different PCR labs: one site using multiplexed PCR (circles) and two sites using individual PCR assays (squares and triangles). Five miRNA of interest are highlighted: miR-451a (red), miR-335 (orange), miR-375 (yellow), miR-218 (green), miR-125b (blue)

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