Fig. 5

Deviation from predicted ratios as a function of dynamic range. Panels A to F correspond to measurement processes A to F described in Results. Each datapoint in panels A to F represents the difference between the observed and predicted log2 ratios plotted against the average observed and predicted log2 signal for each detected miRNA. Open circles correspond to all detectable non-selective miRNA and yellow, blue, and red filled circles correspond to 1-to-1, brain-, and placenta-selective miRNA, respectively. The median and interquartile range (IQR) of the deviation from predicted for all detected miRNA are indicated by the solid and dashed horizontal lines, respectively. The lower limit of acceptable dispersion (determined by a user selectable deviation of ±0.585 log2, see Results) and the maximum detectable value are indicated by the margins of the darker grey areas, respectively. Margins were not assessed in Panel F. Panel F includes data from three different PCR labs: one site using multiplexed PCR (circles) and two sites using individual PCR assays (squares and triangles). Five miRNA of interest are highlighted: miR-451a (red), miR-335 (orange), miR-375 (yellow), miR-218 (green), miR-125b (blue). Limits and range included in the summary table of the dashboard